2PY6

Crystal structure of Methyltransferase FkbM (YP_546752.1) from Methylobacillus flagellatus KT at 2.20 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-29
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837, 0.97926, 0.97891
Spacegroup name	I 2 2 2
Unit cell lengths	73.660, 119.170, 122.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.788 - 2.150
R-factor	0.169
Rwork	0.166
R-free	0.22400
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.747
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.788	29.790	2.230
High resolution limit [Å]	2.150	4.610	2.150
Rmerge	0.097	0.037	0.424
Number of reflections	29426	5449	5583
<I/σ(I)>	7.69	18.8	2.2
Completeness [%]	96.1	94.6	94.6
Redundancy	3.24

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	NANODROP, 0.214M Ammonium nitrate, 14.0% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 277K