2PXX

Human putative methyltransferase MGC2408

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97926
Spacegroup name	P 1 21 1
Unit cell lengths	35.883, 45.560, 55.102
Unit cell angles	90.00, 105.87, 90.00

Refinement procedure

Resolution	30.000 - 1.300
Rwork	0.210
R-free	0.22600
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.317
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.000	70.000	1.350
High resolution limit [Å]	1.300	2.800	1.300
Rmerge	0.096	0.068	0.280
Number of reflections	40375
<I/σ(I)>	8.2
Completeness [%]	96.2	99.7	74.1
Redundancy	6.2	6.9	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	291	28% PEG3350, 0.1 M Ammonium sulfate, 0.1 M Bis-Tris, pH 5.5, VAPOR DIFFUSION, temperature 291K
1	VAPOR DIFFUSION	5.5	291	28% PEG3350, 0.1 M Ammonium sulfate, 0.1 M Bis-Tris, pH 5.5, VAPOR DIFFUSION, temperature 291K