2PXT
Variant 15 of Ribonucleoprotein Core of the E. Coli Signal Recognition Particle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-11 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.380, 78.520, 32.600 |
| Unit cell angles | 90.00, 94.99, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| Rwork | 0.223 |
| R-free | 0.24540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.4L) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.260 | 39.260 | 1.710 |
| High resolution limit [Å] | 1.650 | 3.550 | 1.650 |
| Rmerge | 0.085 | 0.051 | 0.357 |
| Total number of observations | 30878 | 142 | |
| Number of reflections | 29971 | ||
| <I/σ(I)> | 8.9 | 27.6 | 2.4 |
| Completeness [%] | 73.7 | 99.9 | 2.1 |
| Redundancy | 6.38 | 7.25 | 1.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 20Mm NaOH-MES pH 5.6, 200mM KCl, 10% Isopropanol, 5mM Cobalt Hexamine, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 20Mm NaOH-MES pH 5.6, 200mM KCl, 10% Isopropanol, 5mM Cobalt Hexamine, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NaOH-MES | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | Isopropanol | ||
| 4 | 1 | 1 | Cobalt Hexamine | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | NaOH-MES | ||
| 7 | 1 | 2 | KCl | ||
| 8 | 1 | 2 | Isopropanol | ||
| 9 | 1 | 2 | Cobalt Hexamine |
