2PXD
Variant 1 of Ribonucleoprotein Core of the E. Coli Signal Recognition Particle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 2006-02-04 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 131.881, 77.867, 32.428 |
Unit cell angles | 90.00, 94.04, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
Rwork | 0.251 |
R-free | 0.28320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB 1DUL |
Data reduction software | d*TREK |
Data scaling software | d*TREK (9.4L) |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.930 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.150 | 0.595 |
Total number of observations | 8183 | |
Number of reflections | 22094 | |
<I/σ(I)> | 5.2 | 2.2 |
Completeness [%] | 99.6 | 98.3 |
Redundancy | 4.11 | 3.72 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 20Mm NaOH-MES pH 5.6, 200mM KCl, 10% Isopropanol, 5mM Cobalt Hexamine, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | NaOH-MES | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | Isopropanol | ||
4 | 1 | 1 | Cobalt Hexamine | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | NaOH-MES | ||
7 | 1 | 2 | KCl | ||
8 | 1 | 2 | Isopropanol | ||
9 | 1 | 2 | Cobalt Hexamine |