2PWB
Crystal structure of the complex of proteinase K with coumarin at 1.9 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-14 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5432 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.300, 68.300, 108.384 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.740 - 1.900 |
| R-factor | 0.14397 |
| Rwork | 0.142 |
| R-free | 0.17331 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ic6 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.461 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.510 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 19742 | |
| Completeness [%] | 99.5 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | CaCl2 & NaNO3, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
