2PW6
Crystal structure of uncharacterized protein JW3007 from Escherichia coli K12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97243 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 83.940, 83.940, 85.509 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 72.740 - 2.270 |
| R-factor | 0.23616 |
| Rwork | 0.235 |
| R-free | 0.26670 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.703 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SCA2STRUCTURE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.740 | 2.350 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Number of reflections | 16342 | |
| <I/σ(I)> | 18.1 | 5.63 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 20.7 | 20.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 2 microliter drops containing equal volumes of protein concentrate (11.38 mg/ml) in 20mM Tris-HCl, 200mM NaCl, 1mM DTT and resevoir solution containing 0.1M Hepes, 0.8M Sodium acetate, and 0.05M Cadmium sulfate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
