2PUW
The crystal structure of isomerase domain of glucosamine-6-phosphate synthase from Candida albicans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8125 |
| Spacegroup name | I 4 |
| Unit cell lengths | 148.864, 148.864, 102.878 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.550 - 3.151 |
| R-factor | 0.23096 |
| Rwork | 0.230 |
| R-free | 0.27948 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2poc |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.514 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.200 |
| High resolution limit [Å] | 3.151 | 3.151 |
| Number of reflections | 19526 | |
| <I/σ(I)> | 7.7 | 1.7 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 5.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 20% w/v PEG 600, 0.15M KSCN, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
