2PUS

Unprecedented activation mechanism of a non-canonical RNA-dependent RNA polymerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	200
Detector technology	CCD
Collection date	2005-05-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.072
Spacegroup name	P 61 2 2
Unit cell lengths	123.171, 123.171, 362.216
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.890 - 2.400
R-factor	0.23544
Rwork	0.234
R-free	0.26258
Structure solution method	MAD + Molec Replacement
RMSD bond length	0.006
RMSD bond angle	0.922
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP (+ AMoRe)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	67444
<I/σ(I)>	21.4	1.9
Completeness [%]	97.6	81.5
Redundancy	9	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	10-12% PEG 3350, 0.3-0.5M LiNO3, pH 6.5 to 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP		298	10-12% PEG 3350, 0.3-0.5M LiNO3, pH 6.5 to 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP		298	10-12% PEG 3350, 0.3-0.5M LiNO3, pH 6.5 to 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP		298	10-12% PEG 3350, 0.3-0.5M LiNO3, pH 6.5 to 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K