2PUB
CRYSTAL STRUCTURE OF THE LACI FAMILY MEMBER, PURR, BOUND TO DNA: MINOR GROOVE BINDING BY ALPHA HELICES
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Detector | SDMS |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 175.810, 94.870, 81.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.700 |
| Rwork | 0.171 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 20.810 * |
| Phasing software | DIFFERENCE (FOURIER) |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.053 |
| Number of reflections | 18609 |
| <I/σ(I)> | 7.2 |
| Completeness [%] | 99.0 |
| Redundancy | 4.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | Schumacher, M.A., (1994) J.Mol.Biol, 242, 302. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 10 | 1 | 2 | AMMONIUM PHOSPHATE | ||
| 2 | 1 | 1 | NA-K PHOSPHATE | ||
| 3 | 1 | 1 | DTT | ||
| 4 | 1 | 1 | NA CACODYLATE | ||
| 5 | 1 | 1 | PRECIPITATING SOLUTION | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | PEG 4000 | ||
| 8 | 1 | 2 | AMMONIUM SULFATE | ||
| 9 | 1 | 2 | COBALT HEXAMINE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.5 (mM) | |
| 10 | 1 | reservoir | PEG4000 | 25 (%) | |
| 11 | 1 | reservoir | ammonium sulfate | 0.4 (M) | |
| 12 | 1 | reservoir | cobalt hexammine | 50 (mM) | |
| 13 | 1 | reservoir | ammonium phosphate | 0.1 (M) | pH7.5 |
| 2 | 1 | drop | DTT | 1.0 (mM) | |
| 3 | 1 | drop | sodium potassium phosphate | 160 (mM) | pH7.4 |
| 4 | 1 | drop | DNA | 0.6 (mM) | |
| 5 | 1 | drop | sodium cacodylate | 50 (mM) | pH6.9 |
| 6 | 1 | drop | PEG4000 | 25 (%) | |
| 7 | 1 | drop | ammonium sulfate | 0.4 (M) | |
| 8 | 1 | drop | cobalt hexammine | 50 (mM) | |
| 9 | 1 | drop | ammonium phosphate | 0.1 (M) | pH7.5 |
