2PU1
Crystal Structure of the T. brucei enolase complexed with Fluoro-phosphonoacetohydroxamate (FPAH)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-20 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.809, 110.644, 109.006 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.040 - 1.800 |
| R-factor | 0.16452 |
| Rwork | 0.162 |
| R-free | 0.20584 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oep |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.611 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.000 | 1.990 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.056 | 0.435 |
| Number of reflections | 42224 | |
| <I/σ(I)> | 23.3 | 4.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.6 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 10 % (w/v) PEG1000, 0.01 M ZnSO4 or ZnCl2, and 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
