2PTU
Structure of NK cell receptor 2B4 (CD244)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 79.040, 183.268, 59.689 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.380 |
R-factor | 0.22711 |
Rwork | 0.225 |
R-free | 0.26947 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ptt |
RMSD bond length | 0.014 |
RMSD bond angle | 1.550 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.470 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.030 | 0.140 |
Number of reflections | 57316 | |
<I/σ(I)> | 20.9 | 3.5 |
Completeness [%] | 85.1 | 60 |
Redundancy | 1.8 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 2.0 M ammonium dihydrogen phosphate, 0.1 mM Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |