2PSG

REFINED STRUCTURE OF PORCINE PEPSINOGEN AT 1.8 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	105.780, 43.410, 88.580
Unit cell angles	90.00, 91.40, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.164
RMSD bond length	0.016
RMSD bond angle	0.047
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000 *
High resolution limit [Å]	1.800 *
Rmerge	0.058 *
Total number of observations	37575 *
Number of reflections	27251 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.1 *		took James & Sielecki, 1986 from original paper *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservior		1.8 (M)
3	1	reservoir	[N-morpholino]ethane sulphonic acid	50 (mM)