2PS7
Y295F trichodiene synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.91756 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 122.431, 122.431, 151.289 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.350 |
| Rwork | 0.233 |
| R-free | 0.25800 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1jfa |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.109 | 0.574 |
| Number of reflections | 54010 | |
| <I/σ(I)> | 14.1 | 3 |
| Completeness [%] | 98.2 | 97.8 |
| Redundancy | 6.2 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 277 | PEG 8000, SODIUM HEPES, CALCIUM CHLORIDE, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
