2PRY
Apo form of S. cerevisiae orotate phosphoribosyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 93.15 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-07 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 59.791, 59.791, 135.595 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.920 - 2.350 |
| R-factor | 0.239 |
| Rwork | 0.238 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sto |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.065 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.053 | 0.246 |
| Number of reflections | 10718 | |
| <I/σ(I)> | 24.4 | 3.7 |
| Completeness [%] | 98.5 | 84.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293.15 | 26% PEG 6000, 0.2M magnesium acetate, 0.1M Tris HCl, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
