2PRS

Structure and metal binding properties of ZnuA, a periplasmic zinc transporter from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-04-07
Detector	MAR scanner 345 mm plate
Spacegroup name	C 1 2 1
Unit cell lengths	91.377, 78.073, 86.366
Unit cell angles	90.00, 91.37, 90.00

Refinement procedure

Resolution	28.800 - 1.700
R-factor	0.213
Rwork	0.211
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ZnZnuA in space group P212121
RMSD bond length	0.009
RMSD bond angle	1.220
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.800	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	64618
Completeness [%]	97.2	85.5
Redundancy	3.6	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	30 % (w/v) PEG 4000, 5 % isopropanol, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	30 % (w/v) PEG 4000, 5 % isopropanol, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	30 % (w/v) PEG 4000, 5 % isopropanol, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K