Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 298 |
| Collection date | 1994-08-26 |
| Detector | SIEMENS |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.500, 128.500, 116.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.170 - 2.300 |
| R-factor | 0.173 * |
| Rwork | 0.192 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2prj |
| RMSD bond length | 0.016 * |
| RMSD bond angle | 3.100 * |
| Data reduction software | XDS |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.170 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.065 | |
| Total number of observations | 75440 * | |
| Number of reflections | 41448 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 94.7 | 76.8 |
| Redundancy | 1.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | Oikonomakos, N.G., (1985) Biochim. Biophys. Acta, 832, 248. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | DITHIOTHREITOL | ||
| 2 | 1 | 1 | BES | ||
| 3 | 1 | 1 | EDTA | ||
| 4 | 1 | 1 | NAN3 |
