2PR5

Structural Basis for Light-dependent Signaling in the Dimeric LOV Photosensor YtvA (Dark Structure)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-06
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 2
Unit cell lengths	88.846, 91.634, 34.239
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.820 - 1.450
R-factor	0.192
Rwork	0.190
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.190
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	15.000	15.000	1.470
High resolution limit [Å]	1.450	3.910	1.450
Rmerge	0.056	0.031	0.449
Number of reflections	48278
<I/σ(I)>	9.9
Completeness [%]	95.5	96.4	99.9
Redundancy	6.6	6.3	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	0.1 M Na acetate pH 4.6, 18-22 % (w/v) PEG-4000, 75-150 mM ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K