2PQV
Crystal structure of MutT/nudix family protein from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97951 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.321, 64.675, 49.449 |
| Unit cell angles | 90.00, 107.01, 90.00 |
Refinement procedure
| Resolution | 47.300 - 1.630 |
| R-factor | 0.16993 |
| Rwork | 0.167 |
| R-free | 0.22646 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.352 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.690 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.050 | 0.231 |
| Number of reflections | 36665 | |
| <I/σ(I)> | 31.68 | 3.85 |
| Completeness [%] | 99.0 | 91.4 |
| Redundancy | 3.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 291 | 20% PEG3350, 0.2 M Ammonium chloride, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
