2PQR
Crystal structure of yeast Fis1 complexed with a fragment of yeast Caf4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-02 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.408, 46.948, 64.468 |
| Unit cell angles | 90.00, 99.21, 90.00 |
Refinement procedure
| Resolution | 40.400 - 1.880 |
| R-factor | 0.209 |
| Rwork | 0.208 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 2PQN |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.496 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.400 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.073 | 0.424 |
| Number of reflections | 56106 | |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 99.3 | 98.3 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 16% PEG3350, 0.2 M NaH2PO4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
