2PQN
Crystal structure of yeast Fis1 complexed with a fragment of yeast Mdv1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-12 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.172, 57.388, 69.804 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.810 - 2.150 |
| R-factor | 0.20062 |
| Rwork | 0.198 |
| R-free | 0.23306 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y8m |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.495 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.063 | 0.359 |
| Number of reflections | 9834 | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 98.6 | 89.5 |
| Redundancy | 5.7 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 20% PEG3350, 0.1M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
