2PQG
Crystal structure of inactive ribosome inactivating protein from maize (b-32)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-20 |
| Detector | MAC Science DIP-2030 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.234, 75.620, 97.149 |
| Unit cell angles | 90.00, 93.72, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.380 |
| R-factor | 0.2299 |
| Rwork | 0.211 |
| R-free | 0.22990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rtc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.809 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.480 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.118 | 0.342 |
| Number of reflections | 19282 | |
| <I/σ(I)> | 7.1 | 1.6 |
| Completeness [%] | 84.1 | 70.6 |
| Redundancy | 2.9 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 289 | 2M ammonium sulphate, 0.25 M Tris-HCl and 0.1 M sodium acetate tri-hydrate, pH 3.5., VAPOR DIFFUSION, SITTING DROP, temperature 289K |
