2PPX
Crystal structure of a HTH XRE-family like protein from Agrobacterium tumefaciens
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97948, 0.97935 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 70.696, 70.696, 103.736 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.350 - 2.000 |
| R-factor | 0.19831 |
| Rwork | 0.196 |
| R-free | 0.24382 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.450 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.350 | 2.050 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 10649 | |
| <I/σ(I)> | 11.9 | 2 |
| Completeness [%] | 97.5 | 81.3 |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 2M Ammonium sulfate, 0.2M K/Na tartrate, 0.1M Na citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
