2PPW
The crystal structure of uncharacterized Ribose 5-phosphate isomerase RpiB from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.915, 52.416, 85.018 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.130 - 2.010 |
| R-factor | 0.17902 |
| Rwork | 0.177 |
| R-free | 0.21807 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.204 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.130 | 2.070 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.124 | 0.375 |
| Number of reflections | 26774 | |
| <I/σ(I)> | 21.86 | 4.1 |
| Completeness [%] | 99.5 | 91.46 |
| Redundancy | 9.3 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.1M Na acetate pH 4.6, 0.4M Mg formate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
