2POX
Dark state structure of the reversibly switchable fluorescent protein Dronpa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-26 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 72.894, 107.504, 275.663 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.946 |
R-factor | 0.17621 |
Rwork | 0.174 |
R-free | 0.21612 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2iov |
RMSD bond length | 0.011 |
RMSD bond angle | 1.490 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.060 |
High resolution limit [Å] | 1.946 | 1.946 |
Number of reflections | 79474 | 11810 |
<I/σ(I)> | 15.1 | 3 |
Completeness [%] | 99.7 | 98.2 |
Redundancy | 7.5 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 22% PEG 3350, 140mM Mg(NO3)2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |