2POT
tRNA guanine transglycosylase (TGT) E235Q mutant in complex with guanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-21 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.144, 63.692, 71.101 |
| Unit cell angles | 90.00, 92.87, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.2187 |
| Rwork | 0.219 |
| R-free | 0.28580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p0b |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 90611 |
| <I/σ(I)> | 21 |
| Completeness [%] | 97.6 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
