2POI
Crystal structure of XIAP BIR1 domain (I222 form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2002-09-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.28319 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 34.935, 72.963, 81.673 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| Rwork | 0.203 |
| R-free | 0.21300 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.055 | 0.183 |
| Number of reflections | 17377 | |
| <I/σ(I)> | 11.9 | |
| Completeness [%] | 92.6 | 66 |
| Redundancy | 3.6 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 1.5M NaCl, 10% methanol, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
