2PO7

Crystal structure of human ferrochelatase mutant with His 341 replaced by Cys

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-06-20
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	94.049, 87.608, 110.632
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.080 - 2.200
R-factor	0.21
Rwork	0.210
R-free	0.24500
Structure solution method	DIFFERENCE FOURIER
Starting model (for MR)	1hrk
RMSD bond length	0.006
RMSD bond angle	1.210
Data reduction software	DENZO (1.9.1)
Data scaling software	SCALEPACK (1.9.1)
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Rmerge	0.068
Number of reflections	41766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	CRYSTALS WERE GROWN BY HANGING DROP VAPOR DIFFUSION USING 2 MICROLITER DROPS CONTAINING EQUAL VOLUMES OF PROTEIN SOLUTION (45 MG/ML IN 50 MM TRIS MOPS, 0.1M KCL, 1% NA- CHOLATE, 250 MM IMIDAZOLE, PH 8.1) AND PRECIPITANT SOLUTION (0.1 M HEPES PH 7.5, 0.2 M AMMONIUM SULFATE, 34% PEG 400, 0.1 M SODIUM PHOSPHATE)., pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 291K