2PNW
Crystal structure of membrane-bound lytic murein transglycosylase from Agrobacterium tumefaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 88.665, 88.665, 221.434 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.21900 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.302 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.786 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.079 | 0.380 |
| Number of reflections | 41508 | |
| <I/σ(I)> | 27.5 | 6.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.5 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 1.5 M Ammonium sulfate, 100 mM Sodium potassium tartrate, 175 mM Tri-sodium citrate, pH 7.0, VAPOR DIFFUSION, temperature 294K |
