2PNR
Crystal Structure of the asymmetric Pdk3-l2 Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 |
| Unit cell lengths | 96.155, 96.155, 222.984 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.185 |
| Rwork | 0.176 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y8n |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.580 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.097 | 0.422 |
| Number of reflections | 64645 | |
| <I/σ(I)> | 12.1 | 1.5 |
| Completeness [%] | 92.9 | 64 |
| Redundancy | 4.3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | Protein solution: 5mg/ml, 20mM Tris-HCL pH 8.0, 100mM NaCl, 5mM DTT, 2.5% (v/v) ethylen glycol Precipitant solution: 100mM histidine, 30 mM EDTA, 7% (v/v) MPD., VAPOR DIFFUSION, SITTING DROP, temperature 295K |
