2PNE
Crystal Structure of the Snow Flea Antifreeze Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-03 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 16.699, 74.281, 17.687 |
| Unit cell angles | 90.00, 102.20, 90.00 |
Refinement procedure
| Resolution | 17.290 - 0.980 |
| R-factor | 0.141 |
| Rwork | 0.140 |
| R-free | 0.16400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bog |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.674 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 0.980 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Rmerge | 0.059 | 0.250 |
| Number of reflections | 23972 | |
| <I/σ(I)> | 17.9 | 3 |
| Completeness [%] | 90.6 | 46 |
| Redundancy | 3.3 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
