2PN6
Crystal Structure of S32A of ST1022-Gln complex from Sulfolobus tokodaii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2007-03-07 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 103.663, 103.663, 73.539 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 1.440 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e7w |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.490 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.059 | 0.490 |
| Number of reflections | 35897 | |
| Completeness [%] | 98.3 | 84.5 |
| Redundancy | 11.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 20% PPG P400, 30% i-PrOH, 70mM Na3 Citrate, Cymal-7, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
