2PMU
Crystal structure of the DNA-binding domain of PhoP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-08 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 103.884, 101.129, 86.930 |
Unit cell angles | 90.00, 126.72, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.779 |
R-factor | 0.198 |
Rwork | 0.196 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1KGS C-terminal domain |
RMSD bond length | 0.016 |
RMSD bond angle | 1.554 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.840 |
High resolution limit [Å] | 1.779 | 1.779 |
Rmerge | 0.036 | 0.582 |
Number of reflections | 65220 | |
<I/σ(I)> | 18.6 | 2.03 |
Completeness [%] | 94.8 | 96.7 |
Redundancy | 2.9 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.6 | 298 | 1.4 M Na/K phosphate, pH 5.6, 100 mM glycine, VAPOR DIFFUSION, temperature 298K |