2PMT

GLUTATHIONE TRANSFERASE FROM PROTEUS MIRABILIS

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, Hamburg
Beamline	X11
Temperature [K]	295
Collection date	1997-08
Detector	MARRESEARCH
Spacegroup name	P 43
Unit cell lengths	90.885, 90.885, 117.259
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.2086
Rwork	0.209
R-free	0.24380 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007 *
RMSD bond angle	1.300 *
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.147 *	0.382 *
Total number of observations	60878 *
Number of reflections	22464
<I/σ(I)>	7.8	3.1
Completeness [%]	85.8	90
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	PEG8000	22-28 (%)
3	1	reservoir	HEPES	100 (mM)
4	1	reservoir	reduced DSH	10 (mM)