2PMK
Crystal structures of an isolated ABC-ATPase in complex with TNP-ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.05 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 179.682, 34.648, 38.293 |
| Unit cell angles | 90.00, 98.07, 90.00 |
Refinement procedure
| Resolution | 18.950 - 1.600 |
| R-factor | 0.19348 |
| Rwork | 0.192 |
| R-free | 0.22285 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.566 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.046 | 0.404 |
| Number of reflections | 28837 | |
| <I/σ(I)> | 24.3 | |
| Completeness [%] | 97.4 | 79.5 |
| Redundancy | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
