2PM8
Crystal structure of recombinant full length human butyrylcholinesterase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-12-12 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 150.803, 150.803, 142.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.22516 |
| Rwork | 0.222 |
| R-free | 0.29221 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xlw |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.536 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.250 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.129 | 0.545 |
| Number of reflections | 38439 | |
| <I/σ(I)> | 11.6 | 1.6 |
| Completeness [%] | 95.0 | 82.4 |
| Redundancy | 4.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | Ammonium sulphate, pH 8.5, VAPOR DIFFUSION, SITTING DROP |
