2PLK
Crystal structure of lysine/ornithine decarboxylase complexed with cadaverine from Vibrio vulnificus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-15 |
| Detector | SBC-3 |
| Wavelength(s) | 0.979269 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.245, 88.027, 99.272 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.810 - 2.140 |
| R-factor | 0.17791 |
| Rwork | 0.175 |
| R-free | 0.24003 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f3t |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.517 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.107 | 0.429 |
| Number of reflections | 37184 | |
| <I/σ(I)> | 14.6 | 2 |
| Completeness [%] | 90.6 | 87.7 |
| Redundancy | 4.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 277 | 30% PEG-4000, 0.1M Tris HCl pH8.4, 0.2M Magnesium chloride, 0.5% w/v n-octyl-b-D-glucoside , VAPOR DIFFUSION, HANGING DROP, temperature 277K |
