2PLI

Crystal structure of putative Mg2+ and Co2+ transporter(CorC)associated region from Neisseria meningitidis MC58

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97940
Spacegroup name	P 21 21 21
Unit cell lengths	53.718, 67.991, 129.044
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.700
R-factor	0.17854
Rwork	0.177
R-free	0.21539
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.558
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SHELXD
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.075	0.360
Number of reflections	52593
<I/σ(I)>	25.1	2.83
Completeness [%]	99.7	99.5
Redundancy	5.8	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.2M Zinc acetate, 0.1M Imidazole, 10% PEG 8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.2M Zinc acetate, 0.1M Imidazole, 10% PEG 8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.2M Zinc acetate, 0.1M Imidazole, 10% PEG 8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K