2PLF

The structure of aIF2gamma subunit from the archaeon Sulfolobus solfataricus in the nucleotide-free form.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X12
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X12
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-03
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 31 2 1
Unit cell lengths	94.840, 94.840, 166.430
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.900
R-factor	0.222
Rwork	0.222
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	GAMMA SUBUNIT FORM THE AIF2 ALPHA-GAMMA DIMER. PDB ENTRY: 2AHO
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.105
Number of reflections	19573
Completeness [%]	98.7	97
Redundancy	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5	295	natrium malonate dihydrate, glycine,CdCl2., pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K, pH 4.50