2PL2

Crystal structure of TTC0263: a thermophilic TPR protein in Thermus thermophilus HB27

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-04
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97955, 0.979948, 0.971874
Spacegroup name	C 1 2 1
Unit cell lengths	90.070, 71.020, 77.720
Unit cell angles	90.00, 122.97, 90.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.286
Rwork	0.236
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.172
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.058	0.186
Number of reflections	28100	1338
<I/σ(I)>	12.9
Completeness [%]	97.8
Redundancy	3.7	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	289	100mM Na-Acetate (pH 4.5), 1.6M Ammonium Sulfate , VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	4.5	289	100mM Na-Acetate (pH 4.5), 1.6M Ammonium Sulfate , VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	4.5	289	100mM Na-Acetate (pH 4.5), 1.6M Ammonium Sulfate , VAPOR DIFFUSION, HANGING DROP, temperature 289K