2PKM
Crystal structure of M tuberculosis Adenosine Kinase complexed with adenosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-05 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5412 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 71.900, 71.900, 110.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 62.260 - 1.900 |
| R-factor | 0.21935 |
| Rwork | 0.217 |
| R-free | 0.25956 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | apo MTB ADK |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.336 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.660 | 1.949 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.040 | |
| Number of reflections | 24855 | |
| Completeness [%] | 97.8 | 97.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | Crystals were obtained in conditions containing 0.1 M HEPES-Na, pH 7.5 and 1.4 M trisodium citrate dihydrate, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
