2PK2
Cyclin box structure of the P-TEFb subunit Cyclin T1 derived from a fusion complex with EIAV Tat
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9737 |
| Spacegroup name | H 3 |
| Unit cell lengths | 203.830, 203.830, 124.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.670 |
| R-factor | 0.272 |
| Rwork | 0.274 |
| R-free | 0.30600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | |
| High resolution limit [Å] | 2.600 | 2.670 |
| Rmerge | 0.060 | |
| Number of reflections | 54763 | |
| Completeness [%] | 99.0 | |
| Redundancy | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 283 | 1.9M Ammonium Sulfate, 5% Polyethylene Glycol 400 and 50 mM MES pH6.0, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
