2PJD

Crystal structure of 16S rRNA methyltransferase RsmC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	123.939, 51.503, 73.330
Unit cell angles	90.00, 121.52, 90.00

Refinement procedure

Resolution	15.000 - 2.100
R-factor	0.256
Rwork	0.204
R-free	0.25600
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.110
High resolution limit [Å]	2.100	2.040
Rmerge	0.081
Number of reflections	25390
<I/σ(I)>	12.9	15.2
Completeness [%]	97.0	93.5
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	25% PEG MME 5000, 0.1M Tris-HCl pH8.5, 0.2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K