2PI7

Structure of the catalytic domain of the chick retinal neurite inhibitor-Receptor Protein Tyrosine Phosphatase CRYP-2/cPTPRO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-09-05
Detector	MAR scanner 345 mm plate
Spacegroup name	P 31 2 1
Unit cell lengths	68.316, 68.316, 245.018
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.560 - 2.590
Rwork	0.227
R-free	0.28690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rpm
RMSD bond length	0.014
RMSD bond angle	1.800
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.560	2.730
High resolution limit [Å]	2.590	2.590
Rmerge	0.116	0.345
Number of reflections	21113
<I/σ(I)>	10	3.3
Redundancy	3.6	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	15% PEG 10000, 0.6M Ammonium Nitrate, 125mM NaCl, 25mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K