2PI7
Structure of the catalytic domain of the chick retinal neurite inhibitor-Receptor Protein Tyrosine Phosphatase CRYP-2/cPTPRO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-09-05 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 68.316, 68.316, 245.018 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.560 - 2.590 |
| Rwork | 0.227 |
| R-free | 0.28690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rpm |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.800 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.560 | 2.730 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.116 | 0.345 |
| Number of reflections | 21113 | |
| <I/σ(I)> | 10 | 3.3 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 15% PEG 10000, 0.6M Ammonium Nitrate, 125mM NaCl, 25mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
