2PH7

Crystal structure of AF2093 from Archaeoglobus fulgidus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-31
Detector	MARRESEARCH
Wavelength(s)	0.97240
Spacegroup name	F 2 3
Unit cell lengths	206.536, 206.536, 206.536
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.400 - 2.400
R-factor	0.23225
Rwork	0.230
R-free	0.27143
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.341
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SGXPRO
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.400	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	28734
<I/σ(I)>	39.3	2.08
Completeness [%]	99.9	99.8
Redundancy	6.7	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Modified Microbatch	4.9	291	USING 1.0 MICROLITER DROPS CONTAINING EQUAL VOLUMES OF PROTEIN CONCENTRATE (12 mg/mL) AND SOLUTION CONTAINING 20% PEG 4000, 0.05M SODIUM CHLORIDE, 0.05M LITHIUM SULFATE, 0.1M SODIUM ACETATE pH 4.9, Modified Microbatch, temperature 291K