2PH1

Crystal structure of nucleotide-binding protein AF2382 from Archaeoglobus fulgidus, Northeast Structural Genomics Target GR165

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-27
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97950
Spacegroup name	P 21 21 2
Unit cell lengths	66.937, 78.186, 49.597
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.600 - 2.700
R-factor	0.217
Rwork	0.217
R-free	0.27800
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SnB
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.600	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.086	0.413
Number of reflections	13903
<I/σ(I)>	18.54	5.08
Completeness [%]	100.0	100
Redundancy	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH UNDER OIL	7.5	293	Protein solution: 20 mM Tris-HCl pH 7.5, 100 mM Sodium chloride, 5 mM DTT. Precipitant solution: 100 mM HEPES pH 7.5, 55% MPD, MICROBATCH UNDER OIL, temperature 293K