2PH0
Crystal structure of the Q6D2T7_ERWCT protein from Erwinia carotovora. NESG target EwR41.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97960 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 132.584, 38.736, 58.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.760 - 1.850 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.24300 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.056 | 0.451 |
| Number of reflections | 49308 | |
| <I/σ(I)> | 22.38 | 3.9 |
| Completeness [%] | 99.1 | 97.8 |
| Redundancy | 7 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 291 | 40% PEG 1000, 0.1M Ammonium nitrate, 0.1M MOPS, pH 7.0, Microbatch, temperature 291K |
