2PGI

THE CRYSTAL STRUCTURE OF PHOSPHOGLUCOSE ISOMERASE-AN ENZYME WITH AUTOCRINE MOTILITY FACTOR ACTIVITY IN TUMOR CELLS

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	1997-07-25
Detector	RIGAKU RAXIS II
Spacegroup name	I 2 2 2
Unit cell lengths	75.100, 93.730, 171.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.185
Rwork	0.185
R-free	0.25800 *
Structure solution method	MIRAS
RMSD bond length	0.012
RMSD bond angle	26.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.700	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.070	0.362 *
Total number of observations	104060 *
Number of reflections	24024
<I/σ(I)>	12	3.2
Completeness [%]	93.9	83.4
Redundancy	4.3	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	(mg/ml)
2	1	drop	6-phosphogluconate	(mM)
3	1	drop	phosphate	(mM)
4	1	reservoir	phosphate	(mM)
5	1	reservoir	ammonium phosphate	(M)