2PGF
Crystal structure of adenosine deaminase from Plasmodium vivax in complex with adenosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-28 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97943 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 143.962, 146.608, 50.398 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.890 |
| R-factor | 0.15903 |
| Rwork | 0.157 |
| R-free | 0.20061 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2amx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.058 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.970 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.073 | 0.473 |
| Number of reflections | 42835 | |
| <I/σ(I)> | 10.3 | 2.35 |
| Completeness [%] | 99.2 | 94.6 |
| Redundancy | 7.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 33% PEG 20000, 0.1 M TAPS (pH 9.0), 0.1 M Sodium phosphate (monobasic), 16% acetonitrile, 5 mM adenosine, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
