2PFK

THE CRYSTAL STRUCTURE OF UNLIGANDED PHOSPHOFRUCTOKINASE FROM ESCHERICHIA COLI

Experimental procedure

Spacegroup name	C 1 21 1
Unit cell lengths	177.000, 66.400, 154.000
Unit cell angles	90.00, 118.80, 90.00

Refinement procedure

Resolution	100.000 - 2.400
R-factor	0.166
RMSD bond length	0.012
RMSD bond angle	2.000 *
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.400 *	2.400 *
Rmerge	0.057 *
Total number of observations	388304 *
Number of reflections	59481 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	enzyme	10 (mg/ml)
2	1	1	PEG6000	14 (%(w/v))
3	1	1		1.0 (M)
4	1	1	Tris-HCl	50 (mM)