2PFJ

Crystal Structure of T7 Endo I resolvase in complex with a Holliday Junction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-28
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 32 2 1
Unit cell lengths	92.977, 92.977, 124.265
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	11.930 - 3.100
R-factor	0.26848
Rwork	0.267
R-free	0.29776
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Globular domain from pdb file 1FZR
RMSD bond length	0.011
RMSD bond angle	1.893
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.175
High resolution limit [Å]	3.100	3.100
Number of reflections	11577
<I/σ(I)>	14.8	2.8
Completeness [%]	99.4	100
Redundancy	3.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	20 mM CaCl2, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	CaCl2
2	1	1	H2O
3	1	2	CaCl2
4	1	2	H2O